Rapid and simple detection of Phytophthora cactorum in strawberry using a coupled recombinase polymerase amplification–lateral flow strip assay
نویسندگان
چکیده
منابع مشابه
Specific detection of Phytophthora cactorum in diseased strawberry plants using nested polymerase chain reaction
Validated protocols for DNA purification and PCR amplification are reported for detection of Phytophthora cactorum in diseased strawberry plants. To remove PCR inhibitors, necrotic strawberry tissues were soaked in 5% alconox solution for >12 h before DNA extraction, and the extracted genomic DNA was embedded in an agarose gel chamber and subjected to electrophoresis. The purified DNA was ampli...
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ژورنال
عنوان ژورنال: Phytopathology Research
سال: 2021
ISSN: 2524-4167
DOI: 10.1186/s42483-021-00089-8